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Briefly, in the NADH-coupled assay, 14 g (1.2 M) of detergent-purified protein was added to 150 L of reaction buffer containing 50 mM HEPES-KOH pH 8.0, 60 g/mL pyruvate kinase (Roche), 16 mg/mL lactate dehydrogenase (Roche), 4 mM phosphoenolpyruvate (Roche), 0.3 mM NADH, 3 mM ATP, 10 mM MgCl, 0.01% (w/v) LMNG, and 0.001% (w/v) CHS

Its ability to block estrogen where its harmful (breast, hypothalamus) and activate it where its beneficial (bone, liver) makes it one of the most intelligent and clinically validated hormone therapies available today

This means that taking massive, single doses (e.g., 20 grams all at once) does not proportionally increase blood plasma concentrations, as the specific intestinal transporters simply become overwhelmed and cannot process the excess

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